Other
Cys-Thr

Part:BBa_K1787000:Design

Designed by: Eduardo R. Serratos   Group: iGEM15_NAIT_Edmonton   (2015-09-18)


High Cys-Thr protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order for our sequence to be synthesized, we had to intercalate non-polar amino acids between the cysteines and the threonines; these two being of a polar nature.


Source

The DNA sequence encoding for the protein of interest was engineered in a way so as to have a higher Cysteine and Threonine concentration. It does not exist in Nature. It was introduced into BBa_K1321338 (containing a T7 promoter with a RBS).

References